Master or Ph D student Project Cost Discussion
Expenses can vary depending on factors such as research materials, laboratory access, equipment, and travel. We’re here to help you navigate these costs and explore funding options, grants, or tailored solutions to support your research goals. Let’s discuss your project needs and create a plan that aligns with your budget and aspirations.
PCR amplification and Point mutation analysis
Restriction Enzyme Ordering
“Unlocking the blueprint of life, our sequencing platform combines cutting-edge technology with unparalleled accuracy to transform data into discovery. Every sequence tells a story—let us help you decode it.”
Dr Nay
– CEO Crispr Cas Co Ltd
Primer ordering Services
Our primer ordering service provides high-quality, custom-designed primers tailored to your research needs. With fast turnaround times, competitive pricing, and precision in every sequence, we ensure your experiments start on the right note. Whether for PCR, sequencing, or other applications, our team delivers reliable primers to support your scientific success. Place your order today and experience seamless service from design to delivery.
Sequencing Service
Our advanced sequencing service offers accurate, reliable, and high-throughput DNA, RNA, or protein analysis to support your research and discovery goals. Utilizing cutting-edge technology, we deliver fast turnaround times, detailed results, and expert support for projects of any scale. From genomics to transcriptomics, trust us to provide the precision and quality you need to advance your scientific work.
Any Scientific Problem in your Hand
Every scientific challenge is an opportunity for innovation. Whether you’re tackling complex research questions, troubleshooting experiments, or seeking breakthroughs, our team is here to help. With cutting-edge tools, expertise, and a collaborative approach, we turn problems into solutions. Share your challenge with us, and let’s work together to advance science and achieve your goals.
How can I check my primer sequence?
To check your primer sequence, you can use bioinformatics tools such as:
NCBI BLAST: Verify specificity and potential matches in genomic databases.
Primer-BLAST: Design and validate primers for PCR applications.
OligoAnalyzer (IDT): Analyze primer properties like melting temperature (Tm), GC content, and secondary structures.
Geneious or SnapGene: Visualize and validate primer binding sites on your target sequence.
My target band is gone or no more band in my gel photo
If your target band is missing in your gel photo, consider these troubleshooting steps:
Primer Issues: Verify primer design, concentration, and specificity using tools like BLAST or Primer-BLAST.
Template Quality: Ensure your DNA/RNA template is intact, pure, and at the correct concentration.
PCR Conditions: Optimize annealing temperature, cycle number, and Mg²⁺ concentration.
Gel Issues: Check gel concentration, staining, and electrophoresis conditions.
Reagent Problems: Use fresh reagents and ensure proper storage of enzymes and buffers.
Contamination: Avoid cross-contamination and use proper controls.
How can I check RE cutting site?
To check restriction enzyme (RE) cutting sites in your DNA sequence:
Use Bioinformatics Tools:
NEBcutter (from New England Biolabs): Input your sequence to identify RE cutting sites.
SnapGene or Geneious: Visualize and analyze RE sites in your sequence.
Webcutter: An online tool for identifying RE recognition sites.
Reference Enzyme Databases: Check the enzyme’s recognition sequence and cutting pattern (e.g., NEB or Thermo Fisher websites).
Manual Search: Use the “Find” function in a text editor to locate the recognition sequence in your DNA sequence.
Why not getting purified DNA/RNA or Protein?
If you’re struggling to obtain purified DNA, RNA, or protein, consider these common issues:
Sample Quality: Ensure your starting material is fresh, intact, and not degraded.
Lysis Efficiency: Optimize cell or tissue lysis to release maximum nucleic acids or proteins.
Inhibitors: Remove contaminants (e.g., phenol, salts, or proteins) that may interfere with purification.
Protocol Errors: Follow the purification kit instructions carefully, including proper reagent ratios and incubation times.
Column or Bead Issues: Ensure columns or magnetic beads are not clogged or overloaded.
Storage Conditions: Store purified samples at the correct temperature to prevent degradation.